An experimental study was conducted to test different filter membranes for capturing eDNA/eRNA in the context of cost/time effort and cell fractions encountered in nature. For the first time a formal efficiency modelling was applied in eDNA and eRNA research to assist decision-making around an optimized sampling approach.
We are happy to share our recently published comprehensive review on the challenges for using nucleic acids (environmental DNA and RNA) within a marine environment, and the current methods for sample collection, post-sampling capture and concentration of eDNA/eRNA, preservation, and extraction through a biosecurity monitoring lens. In this paper we also present an outlook on how emerging methodologies can shape the future of invasive species detection. This contribution aims to facilitate dialogue and innovation within this sector, and also guide the direction of the further eDNA/eRNA method development and testing within the DETECT research work stream.