To address the current need in molecular surveillance techniques that are easy, fast, specific, and usable with minimal equipment and expertise, we have designed a recombinase polymerase amplification (RPA) assay for species-specific Sabella spallanzanii detection. Recombinase polymerase amplification is an isothermal amplification method, that amplifies target DNA within 20 minutes at a temperature range of 25°C to 45°C, which makes it possible to run assays at body temperature (~37°C), i.e., hand-held. With the introduction of labelled probes and primer, RPA can be used for fluorometry-based quantitative real time amplification. Detection is also possible with lateral flow (LF) strips, that provide easy visual read-outs. The combination of RPA and LF (RPA-LF) strip is especially valuable in resource-limited environments. RPA-LF was previously successfully implemented for multiple disease carrying organisms/viruses, including SARS-CoV-2 or for invasive species like the Northern Pacific Seastar, Asterias amurensis. A simplified RPA protocol was developed and tested with different end-user groups.